Human Toll Like Receptor 7 (TLR7) ELISA Kit

Cat.#:

JL14412

Regulatory Status:

RUO

Alternative:

CD287

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.78-50ng/mL

Sensitivity:

0.4ng/mL

Specificity:

This kit recognizes Human TLR7 in samples. No significant cross-reactivity or interference between Human TLR7 and analogues was observed.

Sample Type:

Tissue homogenates;cell lysates;cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Immunology

Size & Price:

Human A1BG Polyclonal Antibody

Email *

Message *

Please Sign In.

Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| TLR7

Toll-like receptor 7, also known as TLR7, is a protein that is encoded by the TLR7 gene. Orthologs are found in mammals and birds. It is a member of the toll-like receptor (TLR) family and detects single stranded RNA.The TLR family plays an important role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human TLR7 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human TLR7 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	88-96	90-110
1 : 2	Average (%)	93	96
1 : 4	Range (%)	87-108	104-115
1 : 4	Average (%)	98	108